Bioconjugate Techniques by Greg T. Hermanson PDF

By Greg T. Hermanson

ISBN-10: 012342335X

ISBN-13: 9780123423351

ISBN-10: 0123886236

ISBN-13: 9780123886231

Bioconjugate Techniques is the basic consultant to the amendment and crosslinking of biomolecules to be used in examine, diagnostics, and therapeutics. It presents hugely distinctive details at the chemistry, reagent structures, and sensible purposes for developing categorised or conjugate molecules. It additionally describes dozens of reactions with info on hundreds of thousands of commercially on hand reagents and using those reagents for enhancing or crosslinking peptides and proteins, sugars and polysaccharides, nucleic acids and oligonucleotides, lipids, and artificial polymers. Armed with this data and the considerable protocols supplied, readers will shape exact complexes that may be used for detecting, quantifying, and concentrating on vital analytes.
This publication is helping readers make: excessive task antibody-enzymes conjugates, immunotoxins, immunogen complexes, liposome conjugates; in addition to biotinylated molecules, avidin or streptavidin conjugates, colloidal gold categorized proteins, PEG or dextran complexes, categorized oligonucleotide probes, and fluorescently tagged or radiolabeled molecules.

  • This booklet is the 1st to completely seize the full box of bioconjugate chemistry in one volume
  • Serves as a pragmatic advisor to amendment and cross-linking expertise for study, diagnostics, and therapeutics
  • Provides helpful, specified, easy-to-follow, step by step protocols
  • Contains easy-to-read, and easy-to-understand key techniques for making bioconjugates of all types
  • Efficiently covers the chemistry of bioconjugation, the most important reagents to be had for amendment and cross-linking, and the appliance of those reagents to the synthesis of hugely lively conjugates
  • Cites over greater than references keyed to thoughts coated within the book
  • Uses greater than six hundred figures to demonstrate bioconjugate reagents, their reactions, and applications
  • Suggests resources for all key reagents

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Bioconjugate Techniques by Greg T. Hermanson

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