By Niall Barron
Focused manuscript at the power use/role of miRNAs in bioprocessing, in particular the construction of advanced proteins in mammalian cells. With that during brain I suggest a draft record of topics/chapters alongside the subsequent strains: Intro on CHO/bioprocessing/engineering demanding situations to set scene, Genomic association, biogenesis and mode of motion, determining miRNA objectives: Computational prediction, transcriptomics, proteomices, UTR research, etc., miRNA expression in chinese language Hamster Ovary cells, miRNAs as engineering goals: pathway manipulation to affect bioprocess phenotypes, miRNAs as biomarkers, Detection equipment: Northern, PCR, hybridization arrays, subsequent Gen Seq, Manipulation of expression in cultured cells: Transient/stable disregulation, Knockout.
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MicroRNAs as Tools in Biopharmaceutical Production by Niall Barron